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Catalog Number | orb1529516 |
---|---|
Category | Antibodies |
Description | PRMT5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Immunogen | between 580 and C-term |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-PRMT5 antibody A300-849A.; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | PRMT5 |
UniProt ID | O14744 |
NCBI | NP_006100.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | protein arginine N-methyltransferase 5; SKB1Hs; SK Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human PRMT5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PRMT5 used at a dilution of 1:200 (1µg/ml).
Detection of human and mouse PRMT5 by WB (h&m) and IP (h).Samples: HeLa, HEK293T and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PRMT5 antibody used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, IF, IH, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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