PRKCB Antibody

• Catalog Number: orb1263536
• Category: Antibodies
• Description: PRKCB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PKC beta1/2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-334 amino acids from the Central region of human PKC beta1/2.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 77 kDa
• Target: PRKCB
• UniProt ID: P05771
• NCBI: P05771
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Protein kinase C beta type, PKC-B, PKC-beta, PRKCB
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:2000
• Expiration Date: 12 months from date of receipt.

Antibody staining PKC beta1/2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: human brain lysate Lane 3: mouse brain lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of PRKCB using PKC beta1/2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCB gene (Lane 2).