PRKCA Antibody

• Catalog Number: orb1261306
• Category: Antibodies
• Description: PRKCA Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: [45F9]
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human PRKCA
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 77kDa
• Target: PRKCA
• UniProt ID: P17252
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, P
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PRKCA antibody (orb1261306). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse brain using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse intestin using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using PRKCA antibody (orb1261306) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of CRC cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of A549 cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of HeLa cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of MCF-7 cells using PRKCA antibody (orb1261306).

Immunofluorescence analysis of A549 cells using Acetyl-Histone H2A-K9 antibody (orb1255246).