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Primate TNF a Protein

Primate TNF a Protein

Catalog Number: orb428888

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb428888
CategoryProteins
DescriptionRecombinant of primate TNF a protein
Form/AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
PurityGreater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility (25°C)It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Protein SequenceVRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
SourceEscherichia Coli
Biological ActivityThe ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
StorageStability: Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Buffer/PreservativesLyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 5% trehalose.
Alternative namesTumor necrosis factor, Cachectin, TNF-alpha, Tumor
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NoteFor research use only
Application notesCytokines And Growth Factors
Expiration Date6 months from date of receipt.