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Catalog Number | orb429420 |
---|---|
Category | Proteins |
Description | Recombinant of primate IL8 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
Purity | Greater than 97.0% as determined by SDS-PAGE. |
Protein Sequence | AVLPRSAKEL RCECIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE PWVQRVVEKF VKRAENQNP |
Application notes | Chemokines |
Source | Escherichia Coli |
Biological Activity | Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 50-150ng/ml, corresponding to a Specific Activity of 6,667-20,000IU/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL-8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized IL-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-8 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
Alternative names | IL-8, CXCL8, Monocyte-derived neutrophil chemotact Read more... |
Note | For research use only |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and HPLC analyses | |
9.14kD |
Greater than 95% as determined by reducing SDS-PAGE. | |
85-95 KDa (reducing condition) |