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Catalog Number | orb429415 |
---|---|
Category | Proteins |
Description | Recombinant of primate IL6 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
Purity | Greater than 97.0% as determined by SDS-PAGE and HPLC analyses. |
Protein Sequence | MAPVLPGEDS KNVAAPHSQP LTSSERIDKH IRYILDGISA LRKETCNRSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEDTCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PEPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSNL RALRQM |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1ng/ml, corresponding to a specific activity of > 1.0 × 10,000,000 IU/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL-6 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized IL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-6 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
Alternative names | IFN-b2, B cell differentiation factor, BCDF, BSF-2 Read more... |
Note | For research use only |
Greater than 95% as determined by reducing SDS-PAGE. | |
85-95 KDa (reducing condition) |
> 97% by SDS-PAGE and HPLC analyses. | |
Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids. | |
Escherichia coli. |