PRDM9 Antibody [RAB-C370], Rabbit IgG

• Catalog Number: orb1671533
• Category: Antibodies
• Description: PRDM9 Antibody [RAB-C370], Rabbit IgG
• Clonality: Recombinant
• Clone Number: RAB-C370
• Tested applications: ChIP, ELISA, IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM9 protein under non-denaturing conditions.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: PRDM9
• UniProt ID: Q9NQV7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Histone-lysine N-methyltransferase PRDM9, PR domai
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of HeLa cells with anti-PRDM9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671533- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Immunofluorescence staining of HEK293 cells with anti-PRDM9. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells permewith 0.15% Triton stained with the chimeric r version (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671533- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-PRDM9 antibody R Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.