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Catalog Number | orb345674 |
---|---|
Category | Antibodies |
Description | PRDM1 BLIMP1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1. |
Concentration | 1.15 mg/mL |
Dilution range | ELISA: 1:65,000 - 1:80,000, IHC: 5µg/mL, WB: 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human BLIMP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is predicted to react with BLIMP1 and its isoforms from human, mouse, rat and other sources based on a 100% homology with the immunizing sequence. This antibody may also recognize other proteins that show high homology/identity with the BLIMP1 sequence. |
Conjugation | Unconjugated |
UniProt ID | Q60636 |
NCBI | 25008939 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Blimp-1 antibody, rabbit anti-PRDM-1 a Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~88 kDa in size corresponding to BLIMP1 by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Raji cell lysate using PRDM1 BLIMP1 antibody
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, WB | |
Bovine, Canine, Mouse, Porcine, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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