PRDM1 BLIMP1 Antibody

SKU: orb345674

Description

PRDM1 BLIMP1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:65,000 - 1:80,000, IHC: 5µg/mL, WB: 1:1,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~88 kDa in size corresponding to BLIMP1 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1.
• Purity: This affinity purified antibody is directed against human BLIMP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is predicted to react with BLIMP1 and its isoforms from human, mouse, rat and other sources based on a 100% homology with the immunizing sequence. This antibody may also recognize other proteins that show high homology/identity with the BLIMP1 sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.15 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Blimp-1 antibody, rabbit anti-PRDM-1 antibody, anti-PRDM1/BLIMP1 antibody, PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Beta-lymphocyte-induced maturation protein 1, positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1, BLIMP1, BLIMP 1, PRDM1

Immunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blots using Biorbyt's affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 at ~88kDa. Lane 1: mock Raji cell lysate. Lane 2: overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate. Lane 3: Raji whole cell nuclear extract. Lane 4: endogenous PRDM1/BLIMP1 in human plasma cell nuclear extract. The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween.

Quick Database Links

UniProt

Q60636

RefSeq:25008939