PRAMEF6 Antibody

• Catalog Number: orb1270180
• Category: Antibodies
• Description: PRAMEF6 Antibody
• Target: PRAMEF6
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This PRAMEF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-423 amino acids from the C-terminal region of human PRAMEF6.
• UniProt ID: Q5VXH4
• MW: 55 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PRAME family member 6, PRAMEF6
• Note: For research use only
• NCBI: Q5VXH4

Western blot analysis in NCI-H460 cell line lysates (35 ug/lane).

PRAMEF6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of PRAMEF6 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.