PRAMEF6 Antibody

SKU: orb1270180

Description

PRAMEF6 Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This PRAMEF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-423 amino acids from the C-terminal region of human PRAMEF6.
• Target: PRAMEF6
• Molecular Weight: 55 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

PRAME family member 6, PRAMEF6

Western blot analysis in NCI-H460 cell line lysates (35 ug/lane).

PRAMEF6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of PRAMEF6 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

PRAMEF6

UniProt

Q5VXH4

RefSeq:Q5VXH4