PRAMEF6 Antibody (C-term)

• Catalog Number: orb1938210
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PRAMEF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-423 amino acids from the C-terminal region of human PRAMEF6.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q5VXH4
• MW: 54852 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB29486
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PRAME family member 6, PRAMEF6
• Note: For research use only
• NCBI: NP_001010889.1

Confocal immunofluorescent analysis of PRAMEF6 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

PRAMEF6 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PRAMEF6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PRAMEF6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

PRAMEF6 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the PRAMEF6 antibody detected the PRAMEF6 protein (arrow).