PPPDE1 antibody

• Catalog Number: orb22547
• Category: Antibodies
• Description: Goat polyclonal antibody to PPPDE1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, WB: 0.5-1 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 21.4
• Target: PPPDE1 / PNAS4
• Entrez: 51029
• Protein Sequence: ELGKEYKGNAYH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti C1orf121 antibody, anti CGI-146 antibody, ant
• Note: For research use only
• Application notes: WB: In transfected HEK293 transiently expressing full-length Human PPPDE1(myc and DYKDDDDK tagged), a band of approx. 30kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.5-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PPPDE1 with C-terminal MYC tag probed with orb22547 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/5000) in lane C. Mock-transfected HEK293 probed with orb22547 (1mg/ml) in Lane B. Pri

orb22547 (10µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.