PPPDE1 Antibody

SKU: orb1249235

Description

PPPDE1 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing full-length Human PPPDE1(myc and DYKDDDDK tagged), a band of approx. 30kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.5-1µg/mL.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 10µg/mL.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ELGKEYKGNAYH
• Target: PPPDE1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PPPDE1, PPPDE peptidase domain containing 1, C1orf121, CGI-146, FAM152A, FLJ21998, PNAS-4, family with sequence similarity 152, member A

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PPPDE1 with C-terminal MYC tag probed with orb1249235 (0.5 ug/ml) in Lane A and probed with anti-MYC Tag (1/5000) in lane C. Mock-transfected HEK293 probed with orb1249235 (1 mg/ml) in Lane B.

orb1249235 (10 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PPPDE1

UniProt

Q9BSY9

RefSeq (Protein):NP_057160.2