PPP2R5A antibody

• Catalog Number: orb214430
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PPP2R5A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPP2R5A. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PPP2R5A
• Entrez: 226849, 5525
• UniProt ID: Q6PD03, Q15172
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Serine/threonine-protein phosphatase 2A 56 kD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PPP2R5A expression in Hela (A), mouse lung (B), mouse muscle (C), rat lung (D), rat spleen (E) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 56 kD)

Immunofluorescent analysis of PPP2R5A staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.