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Catalog Number | orb1249618 |
---|---|
Category | Antibodies |
Description | PPP2R4 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: C-GSLLPIHPVTSG |
Antibody Type | Primary Antibody |
Concentration | 500 ug/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PPP2R4 |
UniProt ID | Q15257 |
NCBI | NP_821070.1, NP_066954.2, NP_821068.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PPP2R4, protein phosphatase 2A activator, regulato Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human, Mouse and Rat Liver lysates and in lysates of cell line HepG2 (calculated MW of 36.8kDa according to NP_066954.2). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249618 (0.5 ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249618 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |