PPP1R10 Rabbit Polyclonal Antibody

• Catalog Number: orb158183
• Category: Antibodies
• Description: PPP1R10 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PPP1R10 (151-250/940aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:25, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 99 kDa
• Target: PPP1R10
• UniProt ID: Q96QC0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PNUTS; CAT 53; CAT53; FB 19; FB19; FB19 protein; M
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPP1R10) polyclonal Antibody, Unconjugated (orb158183) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human HepG2 cell lysates, Primary: Anti-PPP1R10 (orb158183) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 99 kDa, Observed band size: 115 kDa.

Tissue/Cell: Human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ppp1R10 Polyclonal Antibody, Unconjugated (orb158183) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.