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Catalog Number | orb402500 |
---|---|
Category | Antibodies |
Description | PPID Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human PPID recombinant protein (Position: N306-A370). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 38 kDa |
UniProt ID | Q08752 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Peptidyl-prolyl cis-trans isomerase D; PPIase D Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-PPID antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U251 cells using anti-PPID antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of PPID using anti-PPID antibody.Lane 1:human U87 cell;2:rat brain tissue;3:rat liver tissue;4:mouse brain tissue;5:mouse liver tissue.
IF analysis of PPID using anti-PPID antibody. PPID was detected in immunocytochemical section of MCF-7 cells.
IHC analysis of PPID using anti-PPID antibody.PPID was detected in paraffin-embedded section of mouse intestine tissue.
IHC analysis of PPID using anti-PPID antibody. PPID was detected in paraffin-embedded section of human mammary cancer tissue.
IHC analysis of PPID using anti-PPID antibody. PPID was detected in paraffin-embedded section of human rectal cancer tissue.
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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