PPEF1 Rabbit Polyclonal Antibody

SKU: orb312663

Description

PPEF1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PPEF1 (301-400/653aa)
• Target: PPEF1
• Molecular Weight: 75 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PP 7; PP7; PPE1_HUMAN; PPEF 1; PPEF; PPEF-1; Ppef1; PPP 7C; PPP7C; PPP7CA; Protein phosphatase 7, catalytic subunit, alpha isozyme; Protein phosphatase EF hand calcium binding domain 1; Protein phosphatase serine/threonine type with EF hands; Protein phosphatase with EF calcium binding domain; Protein phosphatase with EF calcium-binding domain; Protein phosphatase with EF hand calcium binding domain 1; Serine/threonine protein phosphatase 7; Serine/threonine protein phosphatase with EF hands 1; Serine/threonine-protein phosphatase 7; Serine/threonine-protein phosphatase with EF-hands 1.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PPEF1) Polyclonal Antibody, Unconjugated (orb312663) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PPEF1

UniProt

O14829