POU6F1/BRN5 Rabbit Polyclonal Antibody

SKU: orb312658

Description

POU6F1/BRN5 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human POU6F (25-120/301aa)
• Target: POU6F1
• Molecular Weight: 32 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BRN5; MPOU; TCFB1; 2310038G18Rik; Emb; cns-1; pou[c]; pouc; PO6F1_HUMAN; POU6F1; Brain-specific homeobox/POU domain protein 5 (Brain-5 | Brn-5); mPOU homeobox protein; PO6F1_MOUSE; Octamer-binding transcription factor EMB; Transcription regulatory protein MCP-1; PO6F1_RAT; PO6F1_DANRE; POU domain protein C (POU[C] | ZfPOU[C]);

Paraformaldehyde-fixed, paraffin embedded (mouse intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (POU6F1) Polyclonal Antibody, Unconjugated (orb312658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

POU6F1

UniProt

Q14863