POU2AF1 Antibody / BOB-1

• Catalog Number: orb2641327
• Category: Antibodies
• Description: BOB-1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB-1. Conversely, only some cases of classical Hodgkin lymphoma show BOB-1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB-1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BOB1/2424
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB-1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This BOB-1 antibody is available for research use only.
• UniProt ID: Q16633
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the BOB-1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human spleen with BOB-1 antibody (clone BOB1/2424). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human Raji cell lysate with BOB-1 antibody (clone BOB1/2424). Predicted molecular weight: ~28 kDa (unmodified), 35-40 kDa (ubiquitinated).

Immunofluorescent staining of PFA-fixed human Raji cells with BOB-1 antibody (green, clone BOB1/2424) and Reddot nuclear stain.

Flow cytometry testing of PFA-fixed human Raji cells with BOB-1 antibody (clone BOB1/2424); Red = isotype control, Blue = BOB-1 antibody.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using BOB-1 antibody (clone BOB1/2424). These results demonstrate the foremost specificity of the BOB1/2424 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free BOB-1 antibody (clone BOB1/2424) as confirmation of integrity and purity.