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POU2AF1 Antibody / BOB-1
Catalog Number: orb1826018
| Catalog Number | orb1826018 |
|---|---|
| Category | Antibodies |
| Description | BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | BOB1/7468 |
| Tested applications | IHC-P |
| Reactivity | Human |
| Isotype | Mouse IgG |
| Immunogen | A recombinant partial protein (within amino acids 148-255) from the human protein was used as the immunogen for the POU2AF1 antibody. |
| Antibody Type | Primary Antibody |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Conjugation | Unconjugated |
| Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
| Hazard Information | This POU2AF1 antibody is available for research use only. |
| UniProt ID | Q16633 |
| Storage | Aliquot the POU2AF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human tonsil tissue with BOB1 antibody (clone BOB1/7468). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with BOB1 antibody (clone BOB1/7468). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC testing of FFPE human placental tissue with BOB1 antibody (clone BOB1/7468) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free BOB1 antibody (clone BOB1/7468) as confirmation of integrity and purity.
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