Polysialic acid antibody

• Catalog Number: orb348875
• Category: Antibodies
• Description: Mouse monoclonal antibody to Polysialic acid
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 735
• Tested applications: FC, IB, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Homopolymers of α2,8-linked sialic acid of Escherichia coli K1.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Polysialic acid
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PSA; N-CAM; neural cell adhesion molecule; polySA
• Note: For research use only
• Application notes: Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).
• Expiration Date: 12 months from date of receipt.

References

Bitter-Suermann, D. et al. Monoclonal antibodies to polysialic acid reveal epitope sharing between invasive pathogenic bacteria, differentiating cells and tumor cells Immunol Res, 6, 225-237 (1987)

PubMed: 2448401

Reactivity

Mouse