Polycystin 1 Rabbit Polyclonal Antibody

• Catalog Number: orb13652
• Category: Antibodies
• Description: Polycystin 1 Rabbit Polyclonal Antibody
• Target: PKD1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Polycystin 1 (131-230/4303aa)
• UniProt ID: P98161
• RRID: AB_10749123
• MW: 460 kDa
• Tested applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Autosomal dominant polycystic kidney disease prote
• Research Area: Calcium Channels, Cancer Research, Cell Biology, E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ren, Jian-Gang et al. Down-regulation of polycystin in lymphatic malformations: possible role in the proliferation of lymphatic endothelial cells Hum Pathol, 65, 231-238 (2017)

PubMed: 28552828

Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Polycystin 1 antibody (orb13652), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: SH-Sy5Y. Primary Antibody (green line): Rabbit Anti-Polycystin 1 antibody (orb13652), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.