POLRMT Antibody

• Catalog Number: orb1672346
• Category: Antibodies
• Description: POLRMT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine, Feline, Monkey, Primate
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-POLRMT antibody (orb1672346) was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human POLRMT. The immunogen is located within the last 50 amino acids of POLRMT.
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 139kD Observed: 139 kD
• Target: POLRMT
• UniProt ID: O0411
• NCBI: O0411.2
• Storage: POLRMT antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: POLRMT Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DNA-directed RNA polymerase, mitochondrial, MtRPOL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

WB Validation in Human Cell Lines. Loading: 15 µg of lysate. Antibodies: POLRMT, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of POLRMT in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of POLRMT in Mouse Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 4 Immunofluorescence Validation of POLRMT in Rat Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with orb1672346 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).