POLR2F Antibody

• Catalog Number: orb1258441
• Category: Antibodies
• Description: POLR2F Antibody
• Target: POLR2F
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human POLR2F (NP_068809.1).
• UniProt ID: P61218
• MW: Observed: 14kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HRBP14.4, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6,
• Research Area: Neuroscience
• Note: For research use only

Western blot analysis of extracts of SW620 cells, using POLR2F antibody (orb1258441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using POLR2F antibody (orb1258441). Blue: DAPI for nuclear staining.