POLR2E Antibody

• Catalog Number: orb1258666
• Category: Antibodies
• Description: POLR2E Antibody
• Target: POLR2E
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2).
• UniProt ID: P19388
• MW: Observed: 26kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DNA-directed RNA polymerases I, II, and III subuni
• Research Area: Neuroscience
• Note: For research use only

Western blot analysis of extracts of various cell lines, using POLR2E antibody (orb1258666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human stomach cancer using POLR2E antibody (orb1258666) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using POLR2E antibody (orb1258666).