POLR2A antibody

• Catalog Number: orb607632
• Category: Antibodies
• Description: Mouse monoclonal antibody to RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CTD 8A7
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse / IgM, kappa
• Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Conjugation: Unconjugated
• MW: 192-253kDa
• Target: POLR2A
• Entrez: 5430
• UniProt ID: P24928
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: DNA-directed RNA polymerase II largest subunit RNA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.