POLR1D Antibody

• Catalog Number: orb1256029
• Category: Antibodies
• Description: POLR1D Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 15kDa
• Target: POLR1D
• UniProt ID: Q9Y2S0
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: POLR1D, FLJ20616, MGC9850, POLR1C, RPA16, RPA9, RP
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using POLR1D antibody (orb1256029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Immunofluorescence analysis of A549 cells using POLR1D antibody (orb1256029). Blue: DAPI for nuclear staining.