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POLR1D Antibody
Catalog Number: orb1256029
| Catalog Number | orb1256029 |
|---|---|
| Category | Antibodies |
| Description | POLR1D Antibody |
| Target | POLR1D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1). |
| UniProt ID | Q9Y2S0 |
| MW | Observed: 15kDa |
| Tested applications | IF, WB |
| Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | POLR1D, FLJ20616, MGC9850, POLR1C, RPA16, RPA9, RP Read more... |
| Note | For research use only |
Western blot analysis of extracts of various cell lines, using POLR1D antibody (orb1256029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Immunofluorescence analysis of A549 cells using POLR1D antibody (orb1256029). Blue: DAPI for nuclear staining.
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POLR1D Rabbit Polyclonal Antibody [orb325415]
WB
Bovine, Canine, Rabbit
Human
Rabbit
Polyclonal
Unconjugated
100 μl 
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