POLR1C Antibody

• Catalog Number: orb1264569
• Category: Antibodies
• Description: POLR1C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This POLR1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-346 amino acids from the C-terminal region of human POLR1C.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: POLR1C
• UniProt ID: O15160
• NCBI: O15160
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: DNA-directed RNA polymerases I and III subunit RPA
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: HepG2 whole cell lysate Lane 7: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.