POLR1B Rabbit Polyclonal Antibody

SKU: orb3094925

Description

The POLR1B Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IF, IHC, and WB applications. This antibody targets the DNA-directed RNA polymerase I subunit RPA2 in Human samples.

Research Area

Cancer Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
• Reactivity: Human
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human POLR1B recombinant protein (Position: H18-V1135).
• Target: DNA-directed RNA polymerase I subunit RPA2
• Molecular Weight: 128 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

POLR1B; DNA-directed RNA polymerase I subunit RPA2; RNA polymerase I subunit 2; DNA-directed RNA polymerase I 135 kDa polypeptide; RPA135

Quick Database Links

Gene Symbol

DNA-directed RNA polymerase I subunit RPA2

UniProt

Q9H9Y6