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Catalog Number | orb1728172 |
---|---|
Category | Antibodies |
Description | POLR1B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human |
Immunogen | E.coli-derived human POLR1B recombinant protein (Position: H18-V1135). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 128 kDa |
UniProt ID | Q9H9Y6 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-POLR1B antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of POLR1B using anti-POLR1B antibody.Lane 1:human K562 cell;2:human HepG2 cell;3:human 293T cell.
IF analysis of POLR1B using anti-POLR1B antibody. POLR1B was detected in an immunocytochemical section of PC-3 cells.
IHC analysis of POLR1B using anti-POLR1B antibody. POLR1B was detected in a paraffin-embedded section of human breast cancer tissue.
IHC analysis of POLR1B using anti-POLR1B antibody. POLR1B was detected in a paraffin-embedded section of human lymphoma tissue.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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