POLK monoclonal antibody (M01), clone 6F2

• Catalog Number: orb2291140
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant POLK.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: POLK (AAH50718, 1 a.a. ~ 472 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP
• Tested applications: ELISA, IF, IHC-P, WB
• Clone Number: 6F2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH50718

Western Blot detection against Immunogen (77.66 KDa).

Detection limit for recombinant GST tagged POLK is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

POLK monoclonal antibody (M01), clone 6F2 Western Blot analysis of POLK expression in Hela S3 NE.

Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2. Lane 1: POLK transfected lysate(54.1 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # orb2291140). GAPDH (36.1 kDa) used as specificity and loading control.