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Catalog Number | orb1258432 |
---|---|
Category | Antibodies |
Description | POLH Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human DNA polymerase eta (NP_006493.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 70kDa |
Target | POLH |
UniProt ID | Q9Y253 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | RAD3RAD30A, XP-V, XPV, DNA polymerase eta, RAD30 h Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DNA polymerase eta antibody (orb1258432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using DNA polymerase eta antibody (orb1258432). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using DNA polymerase eta antibody (orb1258432). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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