DNA polymerase eta antibody

• Catalog Number: orb48495
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DNA Polymerase theta
• Clonality: Polyclonal
• Tested applications: ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human DNA polymerase eta(NP_006493.1).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Conjugation: Unconjugated
• MW: 80KDa
• UniProt ID: Q9Y253
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
• Alternative names: Anti-DKFZp781A0112 antibody, anti-DNA polymerase e
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Pan, Qi et al. Knockdown of POLQ interferes the development and progression of hepatocellular carcinoma through regulating cell proliferation, apoptosis and migration Cancer Cell Int, 21, 482 (2021)

PubMed: 34517891

Applications

WB

Reactivity

Human

Western blot analysis of extracts of various cell lines using DNA Polymerase theta antibody

IHC-P staining of human kidney tissue using DNA Polymerase theta antibody (primary antibody dilution at 1:200)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue using DNA Polymerase theta antibody (primary antibody dilution at 1:200)

Immunofluorescense analysis of U2OS cell using DNA Polymerase theta antibody