POLG Rabbit Polyclonal Antibody

SKU: orb156612

Description

POLG Rabbit Polyclonal Antibody

Research Area

Cell Biology, DNA Replication, DNA and RNA, Mitochondrial Biogenesis, mitochondrial transcription

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human POLG (1101-1239/1239aa)
• Target: POLG
• Molecular Weight: 140 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

DNA directed DNA polymerase gamma; DNA polymerase subunit gamma 1; DNA polymerase subunit gamma-1; DPOG1_HUMAN; Mitochondrial DNA polymerase catalytic subunit; Mitochondrial DNA polymerase gamma; POLG 1; POLG A; PolG alpha; PolG-alpha; Polymerase(DNA directed) gamma; PEO; MDP1; SCAE; MIRAS; ORF-Y; POLG1; POLGA; SANDO; MTDPS4A; MTDPS4B; POLGARF;

Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MDP1 / DNA Polymerase gamma Polyclonal Antibody, Unconjugated (orb156612) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MDP1 / DNA Polymerase gamma Polyclonal Antibody, Unconjugated (orb156612) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

POLG

UniProt

P54098