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Catalog Number | orb1528043 |
---|---|
Category | Antibodies |
Description | Pol Lambda Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human, Mouse |
Immunogen | between 525 and 575 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-Pol Lambda antibody A301-640A.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Pol Lambda |
UniProt ID | Q9UGP5 |
NCBI | NP_037406.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | Pol beta2; polymerase (DNA) lambda; polymerase (DN Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody orb1528043 used for IP at 6 µg per reaction.
Detection of human Pol Lambda by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Pol Lambda (Cat. No. orb1528043) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB
Detection of mouse Pol Lambda by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Pol Lambda (Cat. No. orb1528043) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB
IH, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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