Pol Lambda Antibody

• Catalog Number: orb1528042
• Category: Antibodies
• Description: Pol Lambda Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human, Mouse
• Immunogen: between 525 and 575
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-Pol Lambda antibody A301-640A.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Pol Lambda
• UniProt ID: Q9UGP5
• NCBI: NP_037406.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Pol beta2; polymerase (DNA) lambda; polymerase (DN
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody orb1528042 used for IP at 6 µg per reaction.

Detection of mouse Pol Lambda by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Pol Lambda (Cat. No. orb1528042) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB

Detection of human Pol Lambda by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Pol Lambda (Cat. No. orb1528042) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB