PODXL Rabbit Polyclonal Antibody

• Catalog Number: orb11251
• Category: Antibodies
• Description: PODXL Rabbit Polyclonal Antibody
• Target: PODXL
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human PODXL (451-558/558aa)
• UniProt ID: O00592
• RRID: AB_10752913
• MW: 59 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Podocalyxin; PCLP; PCLP1; podocalyxin-like isoform
• Research Area: Cancer Research, Cardiovascular Research, Cell Mar
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-PODXL antibody, Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Antibody (orb11251, 0.2 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of orb11251 at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Kidney (Mouse) lysate at 30 ug, Primary: Anti-PODXL (orb11251) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, ECL excitated the fluorescence, Predicted band size: 59 kD, Observed band size: 59 kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-PODXL (orb11251) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 59 kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PCX Polyclonal Antibody, Unconjugated (orb11251) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.