PMVK Rabbit Polyclonal Antibody

• Catalog Number: orb6533
• Category: Antibodies
• Description: PMVK Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PMVK (15-120/192aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: PMVK
• UniProt ID: Q15126
• RRID: AB_10917532
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: hPMK; HUMPMKI; phosphomevalonate kinase; PMK; PMK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PMVK Polyclonal Antibody, Unconjugated (orb6533) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Sample: Uterus (Mouse) Lysate at 40 ug, Cerebrum (Mouse) Lysate at 40 ug, Heart (Mouse) Lysate at 40 ug, Lymph node (Mouse) Lysate at 40 ug, 293T (Human) Cell Lysate at 30 ug, Primary: Anti-PMVK (orb6533) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 24 kD.