PMEL Antibody

• Catalog Number: orb1274266
• Category: Antibodies
• Description: PMEL Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PMEL/1825R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG, k
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant gp100 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted format: incubate for 30 min at RT (1)Optimal dilution of the recombinant gp100 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PMEL
• UniProt ID: P40967
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted format: incubate for 30 min at RT (1)Optimal dilution of the recombinant gp100 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human melanoma with recombinant gp100 antibody (clone PMEL/1825R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.