PMEL Antibody

• Catalog Number: orb1252706
• Category: Antibodies
• Description: PMEL Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HMB45
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western Blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the gp100 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PMEL
• UniProt ID: P40967
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte
• Note: For research use only
• Application notes: Western Blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the gp100 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of human melanoma (10X) with gp100 antibody (HMB45).

IHC staining of human melanoma (20X) with gp100 antibody (HMB45).

IHC staining of human melanoma with gp100 antibody (HMB45).

IHC staining of testis with gp100 antibody (HMB45).