PLVAP Rabbit Polyclonal Antibody

SKU: orb312806

Description

PLVAP Rabbit Polyclonal Antibody

Research Area

Endothelial, Varscular System

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PLVAP (301-400/442aa)
• Target: PLVAP
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FELS; Fenestrated endothelial linked structure protein; Fenestrated endothelial-linked structure protein; gp68; Hypothetical protein DKFZp667O074; Meca 32 antigen; MECA32 antigen; Panendothelial cell antigen; Plasmalemma vesicle associated protein; Plasmalemma vesicle protein 1; Plasmalemma vesicle-associated protein; Plvap; PLVAP_HUMAN; PV 1; PV-1; PV1; PV1 protein.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (orb312806) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLVAP) Polyclonal Antibody, Unconjugated (orb312806) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

Sample: Lane 1: Mouse Prostate tissue lysates, Lane 2: Mouse Large intestine tissue lysates, Primary: Anti-PLVAP (orb312806) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 62 kDa.

Quick Database Links

Gene Symbol

PLVAP

UniProt

Q9BX97