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PLK4 Antibody
SKU: orb1255543
Featured
Description
Images & Validation
−Item 1 of 9
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3). |
| Target | PLK4 |
| Molecular Weight | Observed: 115kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−PLK4, polo-like kinase 4 (Drosophila), STK18, serine/threonine kinase 18, SAK, Sak, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase
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Western blot analysis of extracts of various cell lines, using PLK4 antibody (orb1255543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (orb1255543) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
PLK4
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
PLK4 Antibody (orb1255543)
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