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Catalog Number | orb1255543 |
---|---|
Category | Antibodies |
Description | PLK4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 115kDa |
Target | PLK4 |
UniProt ID | O00444 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | PLK4, polo-like kinase 4 (Drosophila), STK18, seri Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using PLK4 antibody (orb1255543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (orb1255543) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (orb1255543) at dilution of 1:100 (40x lens).
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Canine, Equine, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, WB | |
Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |