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PLEKHO1 Rabbit Polyclonal Antibody

Catalog Number: orb10417

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10417
CategoryAntibodies
DescriptionPLEKHO1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human PLEKHO1 (51-170/409aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW45 kDa
TargetPLEKHO1
UniProt IDQ53GL0
RRIDAB_10753311
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesPLEKHO1; C-Jun-binding protein; Casein kinase 2-in
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NoteFor research use only
Expiration Date12 months from date of receipt.
PLEKHO1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

PLEKHO1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

PLEKHO1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

PLEKHO1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

PLEKHO1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLEKHO1) Polyclonal Antibody, Unconjugated (orb10417) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

PLEKHO1 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-PLEKHO1 (orb10417) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa.

PLEKHO1 Rabbit Polyclonal Antibody

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CKIP-1 Polyclonal Antibody, Unconjugated (orb10417) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • Anti-PLEKHO1 Antibody [orb1879483]

    FC,  IH,  WB

    Human

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • CKIP-1 Antibody (N-term) [orb1928471]

    FC,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • PLEKHO1 Rabbit Polyclonal Antibody [orb330520]

    WB

    Bovine, Porcine, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • PLEKHO1 Antibody [orb630008]

    ELISA,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg
  • PLEKHO1 Rabbit Polyclonal Antibody (HRP) [orb2115113]

    WB

    Bovine, Human, Porcine, Rat

    Rabbit

    Polyclonal

    HRP

    100 μl