PLCG 2 Rabbit Polyclonal Antibody

SKU: orb6038

Description

PLCG 2 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Lipid Metabolism

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PLCG 2/PLC gamma 2 (645-750/1265aa)
• Target: PLCG2
• Molecular Weight: 147 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLC 2; PLC gamma 2; PLC IV; PLCG2; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; EC 3.1.4.11; phosphoinositide phospholipase C; phospholipase C gamma 2; phospholipase C, gamma 2(phosphatidylinositol specific).

Blank control (blue): Hep G2 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-PLCG 2/AF647 Conjugated antibody (orb6038/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HIST3H3) Polyclonal Antibody, Unconjugated (orb10805) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PLCG2

UniProt

P16885