PLAUR Rabbit Polyclonal Antibody

• Catalog Number: orb13650
• Category: Antibodies
• Description: PLAUR Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PLAUR (201-300/335aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: PLAUR
• UniProt ID: Q03405
• RRID: AB_10752531
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Plasminogen activator, urokinase receptor; Urokina
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Anwar, Sh et al. Immunohistochemical Detection of Urokinase Plasminogen Activator and Urokinase Plasminogen Activator Receptor in Canine Vascular Endothelial Tumours J Comp Pathol, 153, 278-282 (2015)

PubMed: 26286429

Wang, Wei et al. Anti-cancer effect of Aquaporin 5 silencing in colorectal cancer cells in association with inhibition of Wnt/β-catenin pathway Cytotechnology, 70, 615-624 (2018)

PubMed: 29455395

Sample: bone (Mouse) Lysate at 40 ug, Primary: Anti-uPA Receptor (orb13650) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 48 kD.

Sample: Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug, Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug, Primary: Anti-uPA Receptor (orb13650) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 48 kD.

Tissue/Cell: mouse adrenal gland, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated (orb13650) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated (orb13650) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.