Plakophilin 1 Antibody

• Catalog Number: orb386128
• Category: Antibodies
• Description: Recognizes a protein of 75kDa, identified as Plakophilin 1 (PKP1). Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. PKP1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). PKP2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10B2
• Tested applications: FACS, IF, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: A N-terminal human recombinant protein fragment was used as the immunogen for the Plakophilin 1 antibody. Its epitope maps to between amino acids 1-27 of human PKP1.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Plakophilin 1 antibody is available for research use only.
• UniProt ID: Q13835
• Storage: Store the Plakophilin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Plakophilin 1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human U87(lane 1), HeLa(lane 2) and A431(lane 3) cell lysate using Plakophilin 1 antibody