You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1524314 |
---|---|
Category | Antibodies |
Description | Plakoglobin Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 695 and 745 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Plakoglobin |
UniProt ID | P14923 |
NCBI | NP_002221.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | DPIII; catenin (cadherin-associated protein), gamm Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Plakoglobin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Plakoglobin antibody orb1524314 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Plakoglobin was also immunoprecipitated by rabbit anti-Plakoglobin antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Detection of human Plakoglobin by immunohistochemistry.Sample: FFPE section of human prostate hyperplasia. Antibody: Affinity purified rabbit anti-Plakoglobin (Cat. No. orb1524314) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human Plakoglobin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Plakoglobin (Cat. No. orb1524314) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating