gamma Catenin/JUP Antibody

• Catalog Number: orb215939
• Category: Antibodies
• Description: gamma Catenin/JUP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745). Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 81745 MW
• UniProt ID: P14923
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Junction plakoglobin;Catenin gamma;Desmoplakin III
• Note: For research use only
• Application notes: WB: The detection limit for gamma Catenin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of gamma Catenin using anti-gamma Catenin antibody.gamma Catenin was detected in immunocytochemical section of human MCF-7 cell. Biotinylated goat anti-rabbit IgG was used as secondary antibody.

Flow Cytometry analysis of A431 cells using anti-gamma Catenin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of gamma Catenin using anti-gamma Catenin antibody.Lane 1:human 293T cell; 2:human MCF-7 cell; 3:human T47D cell; 4:rat heart tissue.

IF analysis of gamma Catenin using anti-gamma Catenin antibody. gamma Catenin was detected in immunocytochemical section of A431 cells.

IHC analysis of gamma Catenin using anti-gamma Catenin antibody.gamma Catenin was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of gamma Catenin using anti-gamma Catenin antibody.gamma Catenin was detected in paraffin-embedded section of mouse intestine tissue.

IHC analysis of gamma Catenin using anti-gamma Catenin antibody.gamma Catenin was detected in paraffin-embedded section of rat intestine tissue.

IHC analysis of gamma Catenin using anti-gamma Catenin antibody.gamma Catenin was detected in frozen section of rat intestine tissue.