PLA2G4F Antibody

SKU: orb1248912

Description

PLA2G4F Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Predicted Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QTAEEKAFGDFVINR
• Target: PLA2G4F
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLA2G4F, phospholipase A2, group IVF, DKFZp666G192, DKFZp781B229, PLA2G4FZ, cytosolic phospholipase A2 zeta, cPLA2-zeta

Quick Database Links

Gene Symbol

PLA2G4F

UniProt

Q68DD2

RefSeq (Protein):NP_998765.3