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Catalog Number | orb317832 |
---|---|
Category | Antibodies |
Description | PLA2G2A Rabbit Polyclonal Antibody |
Target | PLA2G2A |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Mouse |
Predicted Reactivity | Rat |
Form/Appearance | Liquid |
Concentration | 1mg/ml |
Buffer/Preservatives | Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4. |
Immunogen | KLH conjugated synthetic peptide derived from mouse PLA2G2A (23-100/146aa) |
UniProt ID | P31482 |
MW | 14 kDa |
Tested applications | IF, IHC-Fr, IHC-P |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | PA2GA_HUMAN; phospholipase A2, membrane associated Read more... |
Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Secretory phospholipase A2) Polyclonal Antibody, Unconjugated (orb317832) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.